| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-874-3p |
| Biological function: |
CircTOLLIP may act as a tumor suppressive and stable non-invasive diagnostic biomarker for NSCLC liquid biopsy; it is down-regulated in NSCLC tissues, cell lines, and whole blood, negatively correlated with tumor size, and may restrain malignant processes involving proliferation and apoptosis. |
| Molecular mechanism: |
Possible miRNA sponge mechanism involving absorption of miR-874-3p; the study mainly validates circTOLLIP stability and diagnostic biomarker value. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Down-regulated circulating circTOLLIP could be used as an early diagnostic biomarker in NSCLC, distinguishes NSCLC from benign lung disease and healthy controls, has better diagnostic performance than NSE, CYFR21-1, and CA72-4, and higher circTOLLIP expression was negatively relevant to tumor size. |
| Description: |
This study identifies circTOLLIP, a TOLLIP-derived circRNA, as significantly down-regulated in NSCLC tissues, cell lines, and patient whole blood. Circulating circTOLLIP shows stability in whole blood and diagnostic value for distinguishing NSCLC from benign lung disease and healthy controls, outperforming several traditional tumor markers. The paper also suggests a possible tumor-suppressive role involving miR-874-3p, proliferation, and apoptosis, although the provided evidence mainly supports biomarker validation. |
| Confidence score: |
0.6676 |