| Expression pattern: |
UP |
| Associated gene: |
TRIM44 |
| Associated microRNA: |
miR-1205 |
| Biological function: |
circRAD23B promotes CRC cell proliferation, cell cycle progression, migration, invasion, metastasis-related MMP2/MMP9 expression, and tumor growth, while inhibiting apoptosis; circRAD23B knockdown exerts antitumor effects in vitro and in vivo. |
| Molecular mechanism: |
circRAD23B functions as a miR-1205 sponge to positively regulate TRIM44 expression, thereby promoting CRC progression through the circRAD23B/miR-1205/TRIM44 axis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
High circRAD23B expression was associated with TNM stage and tumor metastasis in CRC patients; circRAD23B was proposed as a reliable target for CRC diagnosis and a potential therapeutic target. |
| Description: |
circRAD23B is up-regulated in colorectal cancer tissues and cells and is associated with TNM stage and metastasis. Functionally, circRAD23B promotes CRC proliferation, cell cycle progression, migration, invasion, and in vivo tumor growth while suppressing apoptosis. Mechanistically, circRAD23B acts as a miR-1205 sponge to up-regulate TRIM44, forming a circRAD23B/miR-1205/TRIM44 axis that supports CRC progression. |
| Confidence score: |
0.7595 |