| Expression pattern: |
DN |
| Associated gene: |
FBXW7, USP28, c-Myc |
| Associated microRNA: |
miR-197-3p |
| Biological function: |
Inhibits TNBC cell proliferation, migration, tumor growth and lung metastasis; acts as a tumor suppressor. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-197-3p to upregulate FBXW7 (ceRNA); additionally encodes FBXW7-185aa protein that increases FBXW7 abundance and induces c-Myc degradation (via USP28 antagonism). |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); metastasis (inhibits); ceRNA regulation (promotes); ubiquitination (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Low expression of circFBXW7 was associated with poorer clinical outcomes; circFBXW7 was negatively correlated with tumor size and lymph node metastasis and was an independent prognostic factor for TNBC patients. |
| Description: |
circFBXW7 is down-regulated in TNBC and functions as a tumor suppressor by inhibiting proliferation, migration, tumor growth and metastasis. Mechanistically, it sponges miR-197-3p to de-repress FBXW7 (ceRNA effect) and also encodes the FBXW7-185aa protein, which increases FBXW7 abundance and promotes c-Myc degradation. Clinically, low circFBXW7 is associated with poorer survival and adverse clinicopathologic features. |
| Confidence score: |
0.783 |