| Expression pattern: |
UP |
| Associated gene: |
CEP55, Ago2 |
| Associated microRNA: |
miR-148b-3p |
| Biological function: |
promotes proliferation, migration and invasion; inhibits apoptosis |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-148b-3p to upregulate CEP55. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
its overexpression was positively associated with increased T stage and lymph node metastasis of the patients; potential diagnostic biomarker and therapeutic target |
| Description: |
circ_0120376 is up-regulated in NSCLC tissues and cell lines and is associated with advanced T stage and lymph node metastasis. It promotes NSCLC cell proliferation, migration and invasion while inhibiting apoptosis by acting as a ceRNA that sponges miR-148b-3p and thereby increases CEP55 expression. |
| Confidence score: |
0.7045 |