circRNA basic information
circBase ID: hsa_circ_0087862
Name: hsa_circ_RAD23B
Synonym: circ-RAD23B
Host Gene: RAD23B
Genomic location(hg19): chr9:110068659-110081160:+
Genomic location(hg38): chr9:107306378-107318879:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC samples; paired adjacent normal tissue specimens; NSCLC tissue samples
Cell lines:

16HBE; H1299; H1581; H358; A549
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CCND2, TIAM1
Associated microRNA: miR-593e3p, miR-653e5p
Biological function: circ-RAD23B functions as an oncogene in NSCLC cells, promotes cell growth, migration and invasion, and inhibits apoptosis.
Molecular mechanism: circ-RAD23B acts as a competing endogenous RNA by sponging miR-593e3p and miR-653e5p, thereby elevating CCND2 and TIAM1 expression, respectively.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis; Cohort Study; Survival Analysis
Clinical significance:

High circ-RAD23B expression was associated with positive lymph node invasion, lower differentiation grade and shorter overall survival, and circ-RAD23B was proposed as a promising biomarker and therapeutic target for NSCLC.
Description:

circ-RAD23B is up-regulated in NSCLC tissues and cell lines and high expression is associated with lymph node invasion, lower differentiation grade and shorter overall survival. Functionally, it promotes NSCLC cell growth, migration and invasion while suppressing apoptosis. Mechanistically, it sponges miR-593e3p and miR-653e5p to increase CCND2 and TIAM1 expression, supporting its potential as a prognostic biomarker and therapeutic target.
Confidence score:

0.7408
Other information
Title:

Circular RNA circ-RAD23B promotes cell growth and invasion by miR-593-3p/CCND2 and miR-653-5p/TIAM1 pathways in non-small cell lung cancer.

Journal: Biochemical and biophysical research communications
Published: 2019
PubMed ID: 30722989
Study type:

combined biological and clinical study
Data availability: -
Code availability: -