| Expression pattern: |
UP |
| Associated gene: |
DDX5, beta-catenin, cyclin D1, c-Myc |
| Associated microRNA: |
- |
| Biological function: |
promotes cardiomyocyte proliferation; inhibits cardiomyocyte apoptosis; mitigates cardiac dysfunction and fibrosis; promotes cardiac repair/regeneration after ischemic injury |
| Molecular mechanism: |
circIGF1R directly binds DDX5, suppresses ubiquitin-dependent proteasomal degradation of DDX5, increases beta-catenin protein level and activates beta-catenin signaling, promoting transcriptional activation of cyclin D1 and c-Myc |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); apoptosis (inhibits); fibrosis (inhibits); ubiquitination (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Transfection; EdU Staining; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; TUNEL; In Vivo Animal Model; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
circIGF1R was elevated in plasma of AMI patients compared with healthy controls |
| Description: |
circIGF1R is up-regulated after myocardial ischemic injury and in AMI patient plasma. It promotes cardiomyocyte proliferation and inhibits apoptosis, enhancing cardiac repair by binding DDX5, stabilizing DDX5 protein via reduced ubiquitin-proteasomal degradation, and activating Wnt/beta-catenin signaling to induce cyclin D1 and c-Myc. |
| Confidence score: |
0.8567 |