| Expression pattern: |
UP |
| Associated gene: |
ITGAV, E-cadherin, Vimentin, Snail |
| Associated microRNA: |
miR-569 |
| Biological function: |
promotes migration, invasion, proliferation, tumor growth and metastasis; promotes EMT progression |
| Molecular mechanism: |
acts as a miRNA sponge (ceRNA) for miR-569 to upregulate ITGAV expression, thereby activating EMT progression |
| Biological pathway or process: |
EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
High hsa_circ_0051040 expression correlates with poor prognosis and has potential diagnostic capability in plasma for HCC (AUC = 0.801). |
| Description: |
hsa_circ_0051040 is up-regulated in HCC tissues/cell lines and promotes proliferation, migration, invasion, and metastasis. It predominantly localizes to the cytoplasm and functions as a ceRNA by sponging miR-569 to upregulate ITGAV, thereby promoting EMT. High expression is associated with poorer survival and its plasma level shows diagnostic potential for HCC. |
| Confidence score: |
0.8846 |