| Expression pattern: |
UP |
| Associated gene: |
ITGA8, Ago2 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
Promotes proliferation, migration, invasion, EMT, and in vivo progression of ovarian cancer; mediates M2 macrophage-derived exosome pro-malignant effects. |
| Molecular mechanism: |
Exosomal circTMCO3 acts as a ceRNA sponge for miR-515-5p, relieving miR-515-5p-mediated repression and upregulating ITGA8. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
circTMCO3 is upregulated in ovarian cancer patients, and high circTMCO3 expression indicates poor survival. |
| Description: |
In ovarian cancer, M2 macrophage-derived exosomes deliver circTMCO3, which promotes malignant phenotypes (proliferation, migration, invasion and EMT) and accelerates in vivo tumor progression. Mechanistically, circTMCO3 acts as a ceRNA sponge for miR-515-5p, leading to ITGA8 upregulation and poorer patient survival associated with high circTMCO3. |
| Confidence score: |
0.8851 |