| Expression pattern: |
DN |
| Associated gene: |
14-3-3 proteins, YWHAB, YWHAH, YWHAQ, YWHAZ, CAMK2D, Tau, p-Tau (S324), p-Tau (S214), GPX4 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits EOC proliferation, migration, and peritoneal metastasis; promotes Tau aggregation-dependent LPO accumulation and ferroptosis. |
| Molecular mechanism: |
Acts as a chaperone molecule by directly binding 14-3-3 proteins, stabilizing them and facilitating recruitment of CAMK2D to phosphorylate Tau at Ser324, forming a hsa_circ_0001546/14-3-3/CAMK2D/Tau complex that drives Tau aggregation, LPO accumulation, and ferroptosis. |
| Biological pathway or process: |
ferroptosis (promotes); lipid metabolism (promotes); metastasis (inhibits); migration (inhibits); invasion (inhibits); proliferation (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Co-IP; IF (Immunofluorescence); In Vivo Animal Model; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0001546 levels were associated with better overall survival and better disease-free survival in the FUSCC cohort. |
| Description: |
hsa_circ_0001546 is down-regulated in epithelial ovarian cancer and functions as a tumor suppressor. It binds 14-3-3 proteins as a chaperone/scaffold to recruit CAMK2D, promoting Tau Ser324 phosphorylation and Tau aggregation, which increases lipid peroxides and induces LPO-dependent ferroptosis, thereby inhibiting EOC peritoneal metastasis. High hsa_circ_0001546 expression is associated with better OS and DFS. |
| Confidence score: |
0.8826 |