| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, CTNNA1 |
| Associated microRNA: |
- |
| Biological function: |
impairs endothelial barrier function and increases endothelial hyperpermeability in sepsis |
| Molecular mechanism: |
binds the RBP EIF4A3 to reduce CTNNA1 mRNA stability and alpha-catenin production |
| Biological pathway or process: |
mRNA stability (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Western Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Transfection; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; ELISA; Survival Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
high expression in sepsis patients, correlated with disease severity and prognosis |
| Description: |
hsa_circ_0074158 is up-regulated in sepsis and aggravates sepsis-induced endothelial barrier dysfunction. It directly binds the RBP EIF4A3 and post-transcriptionally destabilizes its host gene CTNNA1 mRNA, reducing alpha-catenin production and increasing endothelial hyperpermeability. |
| Confidence score: |
0.8382 |