| Expression pattern: |
UP |
| Associated gene: |
RAB27B, VEGFA |
| Associated microRNA: |
miR-607 |
| Biological function: |
promotes angiogenesis; enhances migration, viability, invasiveness and tube formation of endothelial cells; promotes peritoneal dissemination |
| Molecular mechanism: |
Exosomal circPUM1 acts as a ceRNA to sponge miR-607, relieving repression of VEGFA and RAB27B; RAB27B enhances exosome release and VEGFA activates VEGF signaling in endothelial cells to promote angiogenesis. |
| Biological pathway or process: |
angiogenesis (promotes); migration (promotes); invasion (promotes); proliferation (promotes); metastasis (promotes); ceRNA regulation (promotes); VEGF/VEGFR (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circPUM1 was positively correlated with advanced tumor stage and microvessel density (MVD) in ovarian cancer tissues. |
| Description: |
circPUM1 is up-regulated in ovarian cancer and is associated with higher microvessel density. It is packaged into exosomes and transferred to endothelial cells to promote angiogenesis by sponging miR-607 and upregulating VEGFA and RAB27B, enhancing VEGF signaling and exosome release. Silencing circPUM1 with siRNA delivered by pH-responsive MXene nanosheets inhibits angiogenesis and peritoneal dissemination in vitro and in vivo. |
| Confidence score: |
0.8007 |