| Expression pattern: |
UP |
| Associated gene: |
Smad4, Ago2, collagen I, collagen III |
| Associated microRNA: |
miR-34a-5p |
| Biological function: |
promotes cardiac fibroblast migration and collagen I/III expression (atrial fibrosis/structural remodeling in AF) |
| Molecular mechanism: |
ceRNA mechanism: sponges miR-34a-5p to relieve suppression of Smad4 |
| Biological pathway or process: |
fibrosis (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
Elevated plasma levels of hsa_circ_0000118 were independently associated with poor prognosis in patients with AF. |
| Description: |
In atrial fibrillation, hsa_circ_0000118 is upregulated in atrial tissue and higher plasma levels predict worse prognosis. Functionally, it promotes atrial fibrosis/structural remodeling by enhancing cardiac fibroblast migration and collagen I/III production via a ceRNA mechanism involving miR-34a-5p and Smad4. |
| Confidence score: |
0.8198 |