circRNA basic information
circBase ID: hsa_circ_0085361
Name: hsa_circ_TRPS1
Synonym: circTRPS1
Host Gene: TRPS1
Genomic location(hg19): chr8:116599227-116632287:-
Genomic location(hg38): chr8:115587000-115620060:-
Subcellular localization: cytoplasm; exosome; membrane; extracellular
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCa tissues; adjacent normal tissues; tumor tissue; serum; urine; peripheral blood mononuclear cells
Cell lines:

SV-HUC-1; RT4; UM-UC-3; T24; 5637; J82; HEK-293; HEK293T; T24-luc
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: GLS1, Ago2, mTOR pathway, Nrf2, N-cadherin, vimentin, p-mTOR, p-S6K1, 4EBP1, Bcl-2, Ki-67, CD8+ T cells, PD-1, Tim-3, IFN-g, GZMB, perforin
Associated microRNA: miR-141-3p
Biological function: Promotes proliferation, invasion, migration, tumor growth and metastasis of bladder cancer cells; inhibits apoptosis; induces CD8+ T cell exhaustion; supports glutamine metabolism and redox balance in the bladder cancer microenvironment.
Molecular mechanism: Exosome-derived circTRPS1 acts as a ceRNA by sponging miR-141-3p, thereby enhancing GLS1 expression and GLS1-mediated glutamine metabolism, sustaining mTOR pathway activity and ROS scavenging/redox balance, promoting malignant phenotypes and CD8+ T cell exhaustion.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); PI3K/AKT/mTOR (promotes); glutaminolysis (promotes); immune regulation (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Higher circTRPS1 expression correlates with lower overall survival and more aggressive clinicopathological features; urine-derived exosomal circTRPS1 correlates with clinical stage and may serve as a non-invasive biomarker and therapeutic target for BCa.
Description:

This study identifies hsa_circ_0085361/circTRPS1 as an up-regulated exosome-derived circRNA in bladder cancer tissues and serum/urine exosomes. circTRPS1 promotes BCa proliferation, invasion, metastasis, and CD8+ T cell exhaustion by sponging miR-141-3p to enhance GLS1-mediated glutamine metabolism and redox regulation. High circTRPS1 expression is associated with poor overall survival and aggressive clinicopathological features, supporting its potential as a biomarker and therapeutic target.
Confidence score:

0.8879
Other information
Title:

Exosome-derived circTRPS1 promotes malignant phenotype and CD8+ T cell exhaustion in bladder cancer microenvironments.

Journal: Molecular therapy : the journal of the American Society of Gene Therapy
Published: 2022
PubMed ID: 35038580
Study type:

combined biological and clinical study
Data availability: article/Supplemental information; https://doi.org/10. 1016/j.ymthe.2022.01.022
Code availability: -