| Expression pattern: |
UP |
| Associated gene: |
TRIM59, CyclinD1, C-caspase3, GLUT1, LDHA, Ki67 |
| Associated microRNA: |
miR-496 |
| Biological function: |
Circ_0006324 functions as a tumor promoter in NSCLC, promoting cell proliferation, cell-cycle progression, glycolysis and tumor growth, while inhibiting apoptosis. |
| Molecular mechanism: |
Circ_0006324 acts as a miR-496 sponge to positively regulate TRIM59 expression, thereby promoting NSCLC cell growth and glycolysis. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Circ_0006324 was upregulated in NSCLC and related to tumor node metastasis stage and distant metastasis; it might provide a novel target and biomarker for NSCLC therapy. |
| Description: |
Circ_0006324 is upregulated in NSCLC tissues and cells and is associated with advanced TNM stage and distant metastasis. Functionally, it promotes NSCLC cell proliferation, cell-cycle progression, glycolysis and in vivo tumor growth while suppressing apoptosis. Mechanistically, circ_0006324 acts through the miR-496/TRIM59 axis, functioning as a miR-496 sponge to increase TRIM59 expression. |
| Confidence score: |
0.8324 |