| Expression pattern: |
UP |
| Associated gene: |
FOXC1, IRAK3 promoter, MMP7, CCL2, CXCR1, NEDD9, AGO2 |
| Associated microRNA: |
miR-3607 / miR-3607-3p |
| Biological function: |
promotes cell migration, invasion and metastasis; does not affect proliferation, colony formation or cell cycle progression |
| Molecular mechanism: |
ceRNA mechanism / miRNA sponge; positive-feedback loop involving FOXC1 binding to the IRAK3 promoter |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); proliferation (other); cell cycle (other); ceRNA regulation (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Microarray; RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transwell Assay; Wound Healing Assay; CCK8; Colony Formation Assay; Cell Cycle Assay; RIP (RNA Immunoprecipitation); circRIP; Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
predictive of BC recurrence; associated with worse recurrence-free survival; may serve as a potential predictive biomarker and therapeutic target for metastatic BC patients |
| Description: |
circIRAK3 is an oncogenic circRNA upregulated in metastatic breast cancer and associated with recurrence and poor recurrence-free survival. It promotes migration, invasion and lung metastasis by sponging miR-3607, thereby relieving repression of FOXC1 and establishing a positive-feedback loop with IRAK3 transcription. |
| Confidence score: |
0.8738 |