circRNA basic information
circBase ID: hsa_circ_0000515
Name: -
Synonym: circ_0000515
Host Gene: -
Genomic location(hg19): chr14:20811305-20811534:-
Genomic location(hg38): chr14:20343146-20343375:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancer tissues; adjacent normal ones; paracancerous tissues; HCC tissues

Cell lines:

Bel-7402; HepG2; MH-CC88H; Huh7; Hep3B; HL-7702; MHCC88H; HEK293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK10
Associated microRNA: -
Biological function: circ_0000515 promotes HCC cell proliferation, migration, tumor growth and malignant progression; knockdown of circ_0000515 attenuates proliferative and migratory abilities.
Molecular mechanism: circ_0000515 binds MAPK10 and negatively regulates MAPK10, thereby promoting HCC proliferation, migration and tumorigenicity.
Biological pathway or process:

proliferation (promotes); migration (promotes); metastasis (promotes); other pathway/process (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis

Clinical significance:

High circ_0000515 expression predicts advanced tumor staging, lymphatic metastasis, poor progression-free survival, low overall survival, and has diagnostic and prognostic value in HCC.

Description:

circ_0000515 is upregulated in HCC tissues and cell lines and high expression is associated with advanced stage, lymphatic metastasis, and poor survival. Functionally, circ_0000515 promotes HCC cell proliferation, migration and xenograft tumor growth. Mechanistically, it binds and negatively regulates MAPK10, contributing to malignant HCC progression.

Confidence score:

0.7396

Other information
Title:

Circ_0000515 drives the progression of hepatocellular carcinoma by regulating MAPK10.

Journal: European review for medical and pharmacological sciences
Published: 2020
PubMed ID: 32572915
Study type:

combined biological and clinical study

Data availability: -
Code availability: -