circRNA basic information
circBase ID: -
Name: hsa_circ_MAN1A2
Synonym: circMAN1A2(2,3,4,5)
Host Gene: MAN1A2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

paired normal and colon cancer tissues
Cell lines:

HT29; DLD-1; LoVo; HeLa; A549; 293FT; HEK293FT; HCoEpiC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CENPB mRNA, IGF2BP2
Associated microRNA: -
Biological function: promotes cell proliferation and colorectal cancer progression; supports chromosome stability via maintaining CENPB expression
Molecular mechanism: Direct RNA-RNA interaction with CENPB 3′UTR via the back-splicing junction enhances IGF2BP2-mediated CENPB mRNA stability
Biological pathway or process:

proliferation (promotes); mRNA stability (promotes); cell cycle (other)
Detected method:
Q
B
S
Validation methods:

RNA-seq; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; EMSA; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; MTT; Colony Formation Assay; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IF (Immunofluorescence); Bioinformatics Analysis
Clinical significance:

targeting circMAN1A2(2,3,4,5) with LNA represses CRC progression in xenograft models
Description:

circMAN1A2(2,3,4,5) is up-regulated in colorectal/colon cancer and promotes cancer cell proliferation and tumor growth. It directly base-pairs with the CENPB 3′UTR via its BSJ, enhancing IGF2BP2 binding and stabilizing CENPB mRNA, thereby supporting chromosome stability and CRC progression. LNA targeting the BSJ suppresses CRC progression in xenograft models.
Confidence score:

0.8192
Other information
Title:

Direct circMAN1A2(2,3,4,5)-CENPB mRNA interaction regulates cell proliferation and cancer progression.

Journal: Nature communications
Published: 2025
PubMed ID: 41022764
Study type:

combined biological and clinical study
Data availability: GSE274247; PRJCA001113; GSE91445; IPX0010131000; MiOncoCirc database
Code availability: -