| Expression pattern: |
UP |
| Associated gene: |
STAT5a / STAT5A, E-cad, N-cad, Snail |
| Associated microRNA: |
miＲ-339-5p, miＲ-17, miＲ-520h, miＲ-546, miＲ-520H |
| Biological function: |
Promotes prostate cancer progression by enhancing proliferation, migration, invasion and EMT, and inhibiting apoptosis. |
| Molecular mechanism: |
hsa_circ_0005221 mainly localizes in the cytoplasm and acts as a miRNA sponge for miR-339-5p, thereby regulating STAT5A/STAT5a signaling and EMT/apoptosis-related malignant phenotypes in prostate cancer cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for prostate cancer; no prognosis or diagnostic performance was reported. |
| Description: |
hsa_circ_0005221 is up-regulated in prostate cancer tissues and cells. It promotes malignant prostate cancer phenotypes including proliferation, migration, invasion and EMT while suppressing apoptosis, likely by functioning as a cytoplasmic miR-339-5p sponge and regulating the STAT5A/STAT5a pathway. |
| Confidence score: |
0.8119 |