| Expression pattern: |
DN |
| Associated gene: |
Bcl-2, caspase-3, caspase-9, Bax |
| Associated microRNA: |
- |
| Biological function: |
Inhibits glioma cell growth, proliferation, clonogenic capacity and tumor growth in vivo, and facilitates glioma cell apoptosis. |
| Molecular mechanism: |
hsa_circ_0001649 facilitates apoptosis partly through regulation of the Bcl-2/caspase-3 pathway, activating caspase-3/caspase-9, suppressing Bcl-2 and increasing Bax expression. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Down-regulated hsa_circ_0001649 is linked to larger tumor size, advanced WHO grade, lower survival rate and may serve as an independent prognostic predictor for glioma patients after surgery. |
| Description: |
hsa_circ_0001649 is down-regulated in glioma tissues and cell lines, and its decreased expression is associated with larger tumor size, advanced WHO grade and unfavorable overall survival. Functionally, restoring hsa_circ_0001649 suppresses glioma cell proliferation, colony formation and xenograft tumor growth while promoting apoptosis through the Bcl-2/caspase-3 pathway, supporting its role as a tumor-suppressive prognostic and therapeutic target. |
| Confidence score: |
0.6736 |