| Expression pattern: |
UP |
| Associated gene: |
FOXM1, CENPA, CENPB, AGO2 |
| Associated microRNA: |
miR-873-3p |
| Biological function: |
promotes cell proliferation and tumor growth; facilitates cell cycle progression |
| Molecular mechanism: |
circTP63 acts as a ceRNA sponge for miR-873-3p to relieve repression of FOXM1, which then increases CENPA/CENPB and promotes cell cycle progression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Northern Blot; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Cell Cycle Assay; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
circTP63 upregulation is correlated with larger tumor size and higher TNM stage; higher circTP63 level tended to be associated with poor overall survival |
| Description: |
circTP63 (hsa_circ_0068515), derived from TP63, is up-regulated in LUSC and mainly localized in the cytoplasm. It promotes LUSC cell proliferation and tumor growth by sponging miR-873-3p to de-repress FOXM1, which increases CENPA/CENPB and accelerates cell-cycle progression. |
| Confidence score: |
0.8926 |