circRNA basic information
circBase ID: hsa_circ_0004287
Name: hsa_circ_MALAT1
Synonym: circ4287
Host Gene: -
Genomic location(hg19): chr11:65269791-65272066:+
Genomic location(hg38): chr11:65502320-65504595:+
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004975
MONDO name: Alzheimer disease
Disease details: Alzheimer's disease / AD
Disease DO ID:
 10652
Disease MeSH ID:
 D000544
Disease NCIt ID:
 C2866
Disease ICD11 ID:
 1611724421
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

peripheral blood mononuclear cells; PBMCs; skin lesions; biopsy specimens; ear lesions
Cell lines:

THP1; Raw264.7; HaCaT
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: MALAT1, IGF2BP3, S100A8, S100A9, FUS, EIF4A3
Associated microRNA: -
Biological function: inhibits M1 macrophage activation; alleviates skin inflammation; inhibits LPS-induced macrophage activation; promotes degradation of S100A8/S100A9
Molecular mechanism: hsa_circ_0004287 reduces MALAT1 stability by competitively binding IGF2BP3 with MALAT1 in an m6A-dependent manner, leading to ubiquitination degradation of S100A8/S100A9 and suppression of p38/MAPK phosphorylation and macrophage-mediated inflammation; FUS positively and EIF4A3 negatively regulate hsa_circ_0004287 biogenesis under inflammatory conditions
Biological pathway or process:

MAPK (inhibits); macrophage polarization (inhibits); inflammation (inhibits); m6A modification (other); mRNA stability (inhibits); ubiquitination (promotes); immune regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Co-IP; Actinomycin D / DRB Stability Assay; MeRIP / MeRIP-seq; Transfection; ELISA; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; ROC Analysis; Bioinformatics Analysis; RNA-seq
Clinical significance:

hsa_circ_0004287 level in PBMCs could discriminate between AD patients and healthy controls and between psoriasis patients and healthy controls; its expression was negatively correlated with inflammatory cytokines and Scoring Atopic Dermatitis index; it may serve as a therapeutic candidate for AD and psoriasis
Description:

hsa_circ_0004287 is up-regulated in PBMCs of both atopic dermatitis and psoriasis patients and is mainly expressed by macrophages under inflammatory conditions. Functionally, it suppresses M1 macrophage activation and alleviates AD-like and psoriasis-like skin inflammation. Mechanistically, it competitively binds IGF2BP3 to reduce MALAT1 stability in an m6A-dependent manner, promoting S100A8/S100A9 ubiquitination degradation and suppressing p38/MAPK-driven inflammatory activation.
Confidence score:

0.8825
Other information
Title:

Hsa_circ_0004287 inhibits macrophage-mediated inflammation in an N6-methyladenosine-dependent manner in atopic dermatitis and psoriasis.

Journal: The Journal of allergy and clinical immunology
Published: 2022
PubMed ID: 34953789
Study type:

combined biological and clinical study
Data availability: GSE168694; GSE168696
Code availability: -