| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, L1CAM, RBMS1, NFIB pre-mRNA, AGO2 |
| Associated microRNA: |
- |
| Biological function: |
promotes perineural invasion (PNI) in PDAC; enhances tumor-neuron interactions and neurotropism; promotes tumor growth and neural infiltration in vivo |
| Molecular mechanism: |
circNFIB binds IGF2BP3, enhancing L1CAM mRNA stability via the L1CAM 3'-UTR, activating ERK/MAPK signaling; RBMS1 binds NFIB pre-mRNA introns to promote circNFIB biogenesis |
| Biological pathway or process: |
MAPK (promotes); ERK (promotes); invasion (promotes); metastasis (promotes); mRNA stability (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Cohort Study; Clinical Sample Validation |
| Clinical significance: |
circNFIB was associated with poor prognosis and was an independent predictor of poor prognosis in a cohort of 96 PDAC patients; potential therapeutic target |
| Description: |
circNFIB (hsa_circ_0086376) is upregulated in PDAC, especially in samples with high perineural invasion, and high circNFIB indicates poor prognosis. Functionally, circNFIB promotes PDAC perineural invasion in vitro and in vivo. Mechanistically, circNFIB binds the RBP IGF2BP3 to stabilize L1CAM mRNA and activate the ERK/MAPK pathway, while RBMS1 promotes circNFIB biogenesis by binding NFIB pre-mRNA introns. |
| Confidence score: |
0.8974 |