| Expression pattern: |
UP |
| Associated gene: |
IGF1R, AGO2 |
| Associated microRNA: |
miR-6165 |
| Biological function: |
promotes proliferation, migration, invasion, tumor growth and lung metastasis; promotes epithelial-to-mesenchymal transition |
| Molecular mechanism: |
circ_0001459 acts as a ceRNA (miRNA sponge) for miR-6165 to upregulate IGF1R, affecting downstream proteins including p-Akt and EMT markers |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); PI3K/AKT (promotes); apoptosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); TUNEL; H&E Staining; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0001459 was correlated with tumor differentiation and lymph node metastasis; HCC patients with high expression of circ_0001459 displayed shorter overall survival times |
| Description: |
circ_0001459 is upregulated in hepatocellular carcinoma and mainly localizes to the cytoplasm. It promotes HCC proliferation, migration, invasion and metastasis by sponging miR-6165 to relieve repression of IGF1R, thereby enhancing EMT-related and Akt-related signaling; high circ_0001459 associates with worse overall survival. |
| Confidence score: |
0.8793 |