| Expression pattern: |
DN |
| Associated gene: |
HDAC4 |
| Associated microRNA: |
miR-370-3p |
| Biological function: |
Negatively regulates osteogenic differentiation of hASCs and inhibits bone defect repair/bone formation; knockdown promotes osteogenesis. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-370-3p to regulate HDAC4 expression (miR-370-3p targets the 3′-UTR of HDAC4). |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Western Blot; IF (Immunofluorescence); CCK8; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circ_0003204 is downregulated during osteogenic differentiation of human adipose-derived stem cells and acts as a negative regulator of osteogenesis. Mechanistically, it sponges miR-370-3p to modulate HDAC4, and its silencing promotes bone formation/defect repair in a mouse cranial defect model. |
| Confidence score: |
0.7094 |