| Expression pattern: |
UP |
| Associated gene: |
EWS::FLI1, FLI1 3′ UTR |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes cell proliferation and metastasis while inhibiting apoptosis |
| Molecular mechanism: |
circZNF609 acts as a molecular sponge for miR-145-5p, preventing miR-145-5p from binding to the 3′ UTR of EWS::FLI1 and relieving translational repression of EWS::FLI1. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Transfection; MTT; Annexin V/PI Flow Cytometry; Colony Formation Assay; Western Blot; IF (Immunofluorescence); Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In Ewing’s sarcoma cell lines, circZNF609 is highly expressed and predominantly cytoplasmic. It promotes proliferation and tumorigenic behaviors and inhibits apoptosis by sponging miR-145-5p, thereby relieving miR-145-5p-mediated translational repression of EWS::FLI1. |
| Confidence score: |
0.7446 |