| Expression pattern: |
DN |
| Associated gene: |
AGO2, WISP1 |
| Associated microRNA: |
miR-145-3p |
| Biological function: |
Inhibits PCa cell proliferation, invasion and migration; promotes apoptosis; inhibits MMP-2 and MMP-9 expression; promotes activation of Caspase-3, Caspase-8, Caspase-9 and PARP. |
| Molecular mechanism: |
circ_KATNAL1 directly binds miR-145-3p, promotes miR-145-3p expression, enhances miR-145-3p-mediated inhibition of WISP1, and regulates caspase activation and MMP expression through the miR-145-3p/WISP1 pathway. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); migration (inhibits); apoptosis (promotes); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Colony Formation Assay; Wound Healing Assay; ELISA; Bioinformatics Analysis |
| Clinical significance: |
May be an important target for the diagnosis and treatment of PCa. |
| Description: |
circ_KATNAL1 (hsa_circ_0008068), derived from KATNAL1, is down-regulated in prostate cancer cells and mainly localized in the cytoplasm. It directly binds miR-145-3p and functions through the miR-145-3p/WISP1 pathway to suppress proliferation, invasion and migration while promoting apoptosis. The study suggests circ_KATNAL1 acts as a tumor suppressor and may be a potential diagnostic or therapeutic target in PCa. |
| Confidence score: |
0.6874 |