| Expression pattern: |
UP |
| Associated gene: |
PKM2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes LUAD proliferation, migration, invasion, and tumor growth; accelerates aerobic glycolysis; promotes M2 macrophage polarization. |
| Molecular mechanism: |
circP4HB binds PKM2, increases tetrameric PKM2 formation and PKM2 enzymatic activity, thereby enhancing aerobic glycolysis; promotes M2 macrophage phenotype shift via PKM2. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); macrophage polarization (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Mass Spectrometry; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Survival Analysis; RNA-seq |
| Clinical significance: |
High circP4HB expression correlates with advanced TNM stage and lymph node metastasis and is associated with shorter overall survival in LUAD. |
| Description: |
circP4HB (hsa_circ_0046263), derived from P4HB, is up-regulated in LUAD and promotes tumor progression. It mainly localizes to the cytoplasm and directly binds PKM2, increasing PKM2 tetramer formation and enzymatic activity to enhance aerobic glycolysis, and it also promotes M2 macrophage polarization via PKM2; high circP4HB predicts poor overall survival. |
| Confidence score: |
0.8377 |