| Expression pattern: |
UP |
| Associated gene: |
HMGA2, PGE2, p-GSK-3beta, beta-catenin, p-AKT, FASN, ACC1, FABP5 |
| Associated microRNA: |
miR-653-5p |
| Biological function: |
circARL8B promotes breast cancer cell viability, migration, invasion, fatty acid metabolism and tumor growth; circARL8B silencing suppresses these malignant phenotypes in vitro and in vivo. |
| Molecular mechanism: |
circARL8B acts as a sponge for miR-653-5p to regulate HMGA2 expression and may activate the PGE2/PI3K/AKT/GSK-3beta/Wnt/beta-catenin pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); lipid metabolism (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
The findings might facilitate identification of a novel treatment target for patients with BC. |
| Description: |
circARL8B is up-regulated in breast cancer tissues and cells and promotes malignant progression, including cell viability, migration, invasion, fatty acid metabolism and tumor growth. Mechanistically, it sponges miR-653-5p to regulate HMGA2 and may activate the PGE2/PI3K/AKT/GSK-3beta/Wnt/beta-catenin pathway, suggesting potential therapeutic relevance in BC. |
| Confidence score: |
0.7581 |