circRNA basic information
circBase ID: -
Name: hsa_circ_UXS1
Synonym: -
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005150
MONDO name: age-related macular degeneration
Disease details: age-related macular degeneration / AMD
Disease DO ID:
10871
Disease MeSH ID:
-
Disease NCIt ID:
C84391
Disease ICD11 ID:
1514301548
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

aqueous humor; plasma; RPE-choroid complexes

Cell lines:

HUVECs; 293T

In vivo animal model:

other disease animal model

circRNA-disease information
Expression pattern:
UP
Associated gene: placental growth factor (PGF) gene, PGF, mTOR, p70 S6k
Associated microRNA: miR-335-5p
Biological function: CircRNA Uxs1 promotes choroidal neovascularization and angiogenic endothelial cell functions including tube formation, migration, and proliferation; its knockdown suppresses endothelial cell angiogenic activity and alleviates CNV lesions in vivo.
Molecular mechanism: CircRNA Uxs1 acts as a miR-335-5p sponge, increases PGF expression, and activates the mTOR/p70 S6k pathway.
Biological pathway or process:

angiogenesis (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (promotes); PI3K/AKT/mTOR (promotes)

Detected method:
Q
S
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis

Clinical significance:

CircRNA Uxs1 is upregulated in CNV patient specimens and may serve as a promising therapeutic target for CNV.

Description:

CircRNA Uxs1 is upregulated in CNV patient samples and laser-induced CNV mouse models. It promotes CNV-associated angiogenesis, endothelial proliferation, and migration by sponging miR-335-5p, thereby increasing PGF expression and activating the mTOR/p70 S6k pathway. Knockdown of circRNA Uxs1 suppresses endothelial angiogenic functions and reduces CNV lesions, supporting its potential as a therapeutic target.

Confidence score:

0.7938

Other information
Title:

CircRNA Uxs1/miR-335-5p/PGF axis regulates choroidal neovascularization via the mTOR/p70 S6k pathway.

Journal: Translational research : the journal of laboratory and clinical medicine
Published: 2023
PubMed ID: 36690073
Study type:

combined biological and clinical study

Data availability: GSE131646; GSE146887; Supplementary Table 1; Supplementary Table 2; Supplementary Table 3; Supplementary Data; doi:10.1016/j.trsl.2023.01.003
Code availability: -