| Expression pattern: |
UP |
| Associated gene: |
SP1 |
| Associated microRNA: |
miR-1296-5p |
| Biological function: |
promotes breast cancer cell growth/proliferation, migration and invasion; inhibits apoptosis; promotes cell cycle progression; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism: circ_0000520 sponges miR-1296 to regulate SP1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0000520 high expression was associated with poor overall survival; high expression was associated with lymph node metastasis and TNM stage |
| Description: |
In breast cancer, circ_0000520 is up-regulated and functions as an oncogenic circRNA that promotes proliferation, migration, invasion and tumor growth while inhibiting apoptosis. Mechanistically, it is mainly cytoplasmic and acts as a miRNA sponge for miR-1296-5p, thereby relieving miR-1296-mediated repression of SP1 (circ_0000520/miR-1296/SP1 axis). High circ_0000520 expression is associated with worse overall survival. |
| Confidence score: |
0.8256 |