| Expression pattern: |
UP |
| Associated gene: |
NFAT5 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, EMT, tumor growth and immune escape, and inhibits apoptosis. |
| Molecular mechanism: |
circPGPEP1 functions as a ceRNA/miRNA sponge for miR-515-5p, thereby mediating/upregulating NFAT5 expression in CRC. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); immune regulation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Northern Blot; circRIP; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; ELISA; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Cohort Study |
| Clinical significance: |
circPGPEP1 was associated with lymph node metastasis and poor survival prognosis in CRC patients. |
| Description: |
circPGPEP1/hsa_circ_0050102 is up-regulated in colorectal cancer tissues and cell lines and is associated with lymph node metastasis and poor survival prognosis. It promotes CRC malignancy and immune escape by sponging miR-515-5p to mediate NFAT5 expression, thereby enhancing proliferation, migration, invasion, EMT and tumor growth while suppressing apoptosis and anti-tumor T-cell activity. |
| Confidence score: |
0.8889 |