circRNA basic information
circBase ID: hsa_circ_0079993
Name: hsa_circ_POLR2J4
Synonym: -
Host Gene: POLR2J4
Genomic location(hg19): chr7:44043644-44044934:-
Genomic location(hg38): chr7:44004045-44005335:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissue; adjacent normal colorectal tissue

Cell lines:

HCT116; SW620; NCM460; HT29; SW480; DLD-1; HCT-116

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CREB1
Associated microRNA: miR-203a-3p.1, miR-450b-3p, miR-502-5p, miR-1224-3p
Biological function: promotes CRC cell proliferation and tumor growth
Molecular mechanism: ceRNA/miRNA sponge mechanism: circ_0079993 sponges miR-203a-3p.1 to release CREB1
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Sanger Sequencing; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis; Survival Analysis

Clinical significance:

High circ_0079993 expression was associated with metastasis, advanced stage, and reduced overall survival in CRC patients.

Description:

hsa_circ_0079993 (circ_0079993), derived from POLR2J4, is up-regulated in colorectal cancer and functions as an oncogenic circRNA that promotes CRC cell proliferation and xenograft tumor growth. Mechanistically, it acts as a ceRNA by sponging miR-203a-3p.1 to relieve repression of CREB1, and anti-miR-203a-3p.1 reverses the growth inhibition caused by circ_0079993 knockdown.

Confidence score:

0.7618

Other information
Title:

Homo Sapiens Circular RNA 0079993 (hsa_circ_0079993) of the POLR2J4 Gene Acts as an Oncogene in Colorectal Cancer Through the microRNA-203a-3p.1 and CREB1 Axis.

Journal: Medical science monitor : international medical journal of experimental and clinical research
Published: 2019
PubMed ID: 31515467
Study type:

combined biological and clinical study

Data availability: -
Code availability: -