circRNA basic information
circBase ID: hsa_circ_0003998
Name: hsa_circ_ARFGEF2
Synonym: circ_0003998
Host Gene: ARFGEF2
Genomic location(hg19): chr20:47570092-47580435:+
Genomic location(hg38): chr20:48953555-48963898:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent noncancerous tissues; xenograft tumors

Cell lines:

H1299; A549; 16HBE; HUVECs

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CXCL3, METTL3
Associated microRNA: miR-330-5p
Biological function: Promotes NSCLC cell growth, proliferation, angiogenesis, migration, invasion, metastasis, EMT-like marker changes and tumorigenesis; inhibits apoptosis and G0/G1 arrest.
Molecular mechanism: METTL3 promotes circ_0003998 expression through m6A modification; circ_0003998 functions as a ceRNA by sponging miR-330-5p to positively regulate CXCL3 expression, thereby promoting NSCLC malignancy.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes); m6A modification (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Tube Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; Bioinformatics Analysis

Clinical significance:

circ_0003998 might be a new treatment strategy for NSCLC.

Description:

circ_0003998 is up-regulated in NSCLC tissues and cells and functions as an oncogenic circRNA. METTL3 increases circ_0003998 expression through m6A modification, and circ_0003998 promotes NSCLC growth, angiogenesis, migration, invasion and tumorigenesis through the miR-330-5p/CXCL3 axis.

Confidence score:

0.7946

Other information
Title:

METTL3-mediated circ_0003998 serves as oncogene in non-small cell lung cancer through regulating miR-330-5p/CXCL3 axis.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2025
PubMed ID: 40776628
Study type:

combined biological and clinical study

Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -