| Expression pattern: |
UP |
| Associated gene: |
SKP1, Cyclin D1 |
| Associated microRNA: |
miR-1273f |
| Biological function: |
promotes proliferation, migration, and invasion of bladder cancer cells; promotes tumor growth in vivo; promotes G0/G1 phase progression |
| Molecular mechanism: |
ceRNA (miRNA sponge): circGLIS3 binds miR-1273f to upregulate SKP1, which increases Cyclin D1 and promotes cell cycle progression/proliferation |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Transfection; CCK8; EdU Staining; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; RNA Pull-Down; In Vivo Animal Model; Colony Formation Assay; Bioinformatics Analysis |
| Clinical significance: |
ROC curve analysis suggested circGLIS3 may have some diagnostic value in bladder cancer (AUC ~0.662). |
| Description: |
circGLIS3 is up-regulated in bladder cancer tissues/cells and promotes proliferation, migration, invasion and xenograft growth. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-1273f to increase SKP1, which elevates Cyclin D1 and drives G0/G1 cell-cycle progression; it shows limited diagnostic potential (AUC ~0.66). |
| Confidence score: |
0.8175 |