| Expression pattern: |
UP |
| Associated gene: |
HDAC1, Ago2 |
| Associated microRNA: |
miR-874-3p |
| Biological function: |
hsa_circ_0003489 maintains MM cell viability and proliferation, promotes autophagy, inhibits apoptosis, and contributes to bortezomib resistance; its knockdown inhibits viability, proliferation and autophagy, promotes apoptosis, and sensitizes MM cells to bortezomib. |
| Molecular mechanism: |
hsa_circ_0003489 functions as a miR-874-3p sponge and positively regulates HDAC1, thereby controlling the autophagy-apoptosis balance and bortezomib sensitivity in MM cells. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); autophagy (promotes); ceRNA regulation (other); drug resistance (promotes); chemoresistance (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; IF (Immunofluorescence); TUNEL; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
hsa_circ_0003489 is associated with shorter overall survival and progression-free survival, lower responsiveness to bortezomib, and may serve as a diagnostic and prognostic biomarker for MM. |
| Description: |
This study identifies hsa_circ_0003489 as a tumor-promoting circRNA in multiple myeloma. It acts through the miR-874-3p/HDAC1 axis to promote autophagy and proliferation while suppressing apoptosis, thereby reducing sensitivity to bortezomib. Silencing hsa_circ_0003489 shifts MM cells from autophagy toward apoptosis and enhances bortezomib cytotoxicity in vitro and in xenograft models. |
| Confidence score: |
0.7052 |