| Expression pattern: |
UP |
| Associated gene: |
MTDH |
| Associated microRNA: |
miR-124-3p |
| Biological function: |
Promotes endothelial cell viability, tube formation, angiogenesis, and tumor growth in breast cancer context. |
| Molecular mechanism: |
Exosomal circHIPK3 acts as a ceRNA that sponges miR-124-3p, thereby increasing MTDH expression and promoting angiogenesis. |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Might serve as a therapeutic target for anti-angiogenesis therapy for breast cancer. |
| Description: |
In this study, breast cancer cell-derived exosomal circHIPK3 was upregulated and could be transferred to endothelial cells. It promoted endothelial cell proliferation, tube formation, angiogenesis, and tumor growth by sponging miR-124-3p and increasing MTDH expression, suggesting potential value as an anti-angiogenesis therapeutic target in breast cancer. |
| Confidence score: |
0.7799 |