circRNA basic information
circBase ID: hsa_circ_0000231
Name: hsa_circ_ARHGAP12
Synonym: circARHGAP12
Host Gene: ARHGAP12
Genomic location(hg19): chr10:32197099-32199491:-
Genomic location(hg38): chr10:31908171-31910563:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

Saos-2; MG63; Saos-2/Dox; MG63/Dox; hFOB
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: c-Myc mRNA, c-Myc
Associated microRNA: -
Biological function: Promotes doxorubicin resistance, proliferation, and aerobic glycolysis in osteosarcoma cells.
Molecular mechanism: m6A-modified circARHGAP12 binds c-Myc mRNA in an m6A-dependent manner and increases c-Myc mRNA stability.
Biological pathway or process:

glycolysis (promotes); drug resistance (promotes); m6A modification (other); mRNA stability (promotes); proliferation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; Transfection; CCK8; Colony Formation Assay; Bioinformatics Analysis
Clinical significance:

-
Description:

circARHGAP12 is up-regulated in osteosarcoma (including DOX-resistant cells) and promotes doxorubicin resistance, proliferation, and aerobic glycolysis. Mechanistically, its m6A modification enables binding to c-Myc mRNA and increases c-Myc mRNA stability, contributing to chemoresistance-associated metabolic reprogramming.
Confidence score:

0.7716
Other information
Title:

m6A-modified circARHGAP12 promotes the aerobic glycolysis of doxorubicin-resistance osteosarcoma by targeting c-Myc.

Journal: Journal of orthopaedic surgery and research
Published: 2024
PubMed ID: 38178201
Study type:

biological research
Data availability: 10.1186/s13018-023-04502-0
Code availability: -