| Expression pattern: |
UN |
| Associated gene: |
hnRNPL, ERalpha |
| Associated microRNA: |
- |
| Biological function: |
Modulates alternative splicing and promoter usage, regulating CDYL isoform switching in breast cancer cells. |
| Molecular mechanism: |
circCDYL associates with hnRNPL and contributes to chromatin remodeling at CDYL alternative promoters, affecting CDYL isoform selection primarily via alternative promoter usage. |
| Biological pathway or process: |
other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Transfection; ChIP / ChIP-seq; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In MCF-7 breast cancer cells, circCDYL associates with the splicing factor hnRNPL and contributes to widespread alternative splicing changes. circCDYL also regulates its host gene CDYL by influencing isoform selection mainly through alternative promoter usage and chromatin remodeling at CDYL promoter regions, with involvement of ERalpha occupancy. |
| Confidence score: |
0.6475 |