| Expression pattern: |
UP |
| Associated gene: |
STAT3, AGO2 |
| Associated microRNA: |
miR-124-3p |
| Biological function: |
Promotes glioma cell proliferation, invasion, migration and cell cycle progression, and inhibits apoptosis. |
| Molecular mechanism: |
circ-HIPK3 acts as a miR-124-3p sponge in the cytoplasm and upregulates/stabilizes STAT3 expression, forming a circ-HIPK3/miR-124-3p/STAT3 regulatory network. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); migration (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (other); JAK/STAT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Transfection; EdU Staining; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot |
| Clinical significance: |
circ-HIPK3 may exert diagnostic and therapeutic values on glioma. |
| Description: |
hsa_circ_0000284/circ-HIPK3 is up-regulated in human glioma cell lines. It promotes glioma cell proliferation, invasion, migration and cell cycle progression while suppressing apoptosis, mainly by acting as a cytoplasmic sponge for miR-124-3p and thereby increasing STAT3 expression. The study suggests a circ-HIPK3/miR-124-3p/STAT3 axis as a potential mechanism and possible therapeutic target in glioma. |
| Confidence score: |
0.7221 |