circRNA basic information
circBase ID: hsa_circ_0008928
Name: hsa_circ_SATB2
Synonym: circular_0008928 / circSATB2
Host Gene: SATB2
Genomic location(hg19): chr2:200213423-200298237:-
Genomic location(hg38): chr2:199348700-199433514:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum exosomes

Cell lines:

A549; H1299; H661; SK-MES-1; A549/CDDP; H1299/CDDP; 16HBE

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: HK2
Associated microRNA: miR-488
Biological function: Circ_0008928 promotes cisplatin resistance, cell proliferation, migration, invasion, and glycolysis metabolism in CDDP-resistant NSCLC; circ_0008928 knockdown improves CDDP sensitivity and suppresses these malignant phenotypes.
Molecular mechanism: Circ_0008928 acts as a ceRNA sponge for miR-488 to upregulate HK2 expression, thereby regulating CDDP resistance, tumor progression, and glycolysis metabolism.
Biological pathway or process:

ceRNA regulation (promotes); chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; CCK8; Transwell Assay; Western Blot; Bioinformatics Analysis

Clinical significance:

Exosome-based circ_0008928 may serve as a potential biomarker for the diagnosis of NSCLC and is increased in serum exosomes of CDDP-resistant NSCLC patients.

Description:

Circ_0008928 is upregulated in NSCLC cells, CDDP-resistant NSCLC cells, and serum exosomes from CDDP-resistant NSCLC patients. It promotes cisplatin resistance, proliferation, migration, invasion, and glycolysis by sponging miR-488 and increasing HK2 expression, while knockdown restores CDDP sensitivity and suppresses malignant phenotypes. Serum exosomal circ_0008928 is proposed as a potential diagnostic biomarker for NSCLC.

Confidence score:

0.7283

Other information
Title:

Serum Exosomes-Based Biomarker circ_0008928 Regulates Cisplatin Sensitivity, Tumor Progression, and Glycolysis Metabolism by miR-488/HK2 Axis in Cisplatin-Resistant Nonsmall Cell Lung Carcinoma.

Journal: Cancer biotherapy & radiopharmaceuticals
Published: 2023
PubMed ID: 33661058
Study type:

combined biological and clinical study

Data availability: Supplementary Table S1
Code availability: -