circRNA basic information
circBase ID: hsa_circ_0060927
Name: hsa_circ_CYP24A1
Synonym: circ_0060927
Host Gene: CYP24A1
Genomic location(hg19): chr20:52773707-52788209:-
Genomic location(hg38): chr20:54157168-54171670:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0004844
MONDO name: oral mucosa leukoplakia
Disease details: oral leukoplakia / OLK
Disease DO ID:
9655
Disease MeSH ID:
D007972
Disease NCIt ID:
C3187
Disease ICD11 ID:
417308277
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

normal oral mucosal (NOM) tissues; OLK tissues; OSCC tissues; normal oral mucosa tissue samples

Cell lines:

LEUK1; SCC9; SCC25; HEK293T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: TRIM14, AGO2
Associated microRNA: miR-195-5p
Biological function: promotes proliferation and migration and inhibits apoptosis during OLK malignant transformation/carcinogenesis
Molecular mechanism: acts as a ceRNA/miRNA sponge to sequester miR-195-5p and relieve its inhibition on TRIM14; associated with AGO2-containing RISC
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay

Clinical significance:

potential diagnostic biomarker with high diagnostic sensitivity and specificity (ROC/AUC) in carcinogenesis progression of normal mucosa and OLK toward OSCC

Description:

hsa_circ_0060927 is upregulated across NOM→OLK→OSCC and promotes malignant transformation-related phenotypes. It localizes mainly in the cytoplasm and functions as a ceRNA by binding miR-195-5p (via AGO2/RISC), thereby relieving miR-195-5p suppression of TRIM14 and enhancing proliferation and migration while inhibiting apoptosis in LEUK1 cells; it also shows diagnostic potential by ROC analysis.

Confidence score:

0.8748

Other information
Title:

Hsa_circ_0060927 Is a Novel Tumor Biomarker by Sponging miR-195-5p in the Malignant Transformation of OLK to OSCC.

Journal: Frontiers in oncology
Published: 2021
PubMed ID: 35087744
Study type:

combined biological and clinical study

Data availability: GSE131182; GSE131568
Code availability: -