| Expression pattern: |
UP |
| Associated gene: |
SLIT2, TGIF2, eIF4G1, eIF4E, eIF4F complex |
| Associated microRNA: |
miR-548p |
| Biological function: |
Promotes gastric cancer neural invasion in vitro and in vivo; enhances neuritogenesis of dorsal root ganglia and neuronal-cell-dependent migration of gastric cancer cells; downregulates SLIT2 expression in neuronal cells. |
| Molecular mechanism: |
sEV-circVAPA acts through both miRNA sponge and protein-binding mechanisms: it sponges miR-548p to elevate TGIF2 and repress SLIT2 transcription, and binds eIF4G1 to disrupt eIF4G1/eIF4E interaction and suppress SLIT2 translation. |
| Biological pathway or process: |
invasion (promotes); migration (promotes); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis; IF (Immunofluorescence) |
| Clinical significance: |
circVAPA in plasma sEVs and GC tissues may serve as a biomarker for GC neural invasion; higher circVAPA expression in GC tissues predicts poor overall survival. |
| Description: |
hsa_circ_0006990/circVAPA is enriched in plasma small extracellular vesicles and tumor tissues from NI-positive gastric cancer patients and is associated with poor overall survival. Functionally, GC-derived sEV-circVAPA promotes gastric cancer neural invasion by entering neuronal cells, suppressing SLIT2 transcription through the miR-548p/TGIF2 axis, and inhibiting SLIT2 translation through eIF4G1 binding and disruption of the eIF4F complex. |
| Confidence score: |
0.9045 |