| Expression pattern: |
DN |
| Associated gene: |
SP1, NF-kappaB |
| Associated microRNA: |
miR-31-5p |
| Biological function: |
inhibits M1 macrophage activation/polarization and alleviates lung/airway inflammation in severe asthma |
| Molecular mechanism: |
circ-0001875 acts as a miRNA sponge for miR-31-5p to promote SP1 translation, thereby suppressing M1 macrophage polarization via the NF-kappaB signaling pathway |
| Biological pathway or process: |
NF-kappaB (inhibits); macrophage polarization (inhibits); immune regulation (other); inflammation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; ELISA; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circ-0001875 expression levels are associated with asthma severity; reduced in patients with severe asthma |
| Description: |
circ-0001875 is down-regulated in PBMCs of asthma patients (especially severe asthma) and in an OVA-LPS mouse model. It suppresses M1 macrophage polarization and airway/lung inflammation by sponging miR-31-5p, thereby promoting SP1 translation and inhibiting NF-kappaB pathway activation. |
| Confidence score: |
0.7129 |