| Expression pattern: |
UP |
| Associated gene: |
IGF2BP1, HMGA2, ZNF460 |
| Associated microRNA: |
- |
| Biological function: |
Promotes gastric cancer cell migration and metastasis (lung metastasis) and induces EMT-like changes. |
| Molecular mechanism: |
Acts as a molecular scaffold to bind IGF2BP1 and enhance IGF2BP1-dependent stabilization of HMGA2 mRNA; ZNF460 activates NF1 transcription to increase circNF1. |
| Biological pathway or process: |
migration (promotes); metastasis (promotes); EMT (promotes); mRNA stability (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; Western Blot; Dual-luciferase reporter assay; Luciferase Reporter Assay; In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis |
| Clinical significance: |
High circNF1 expression correlates with reduced cumulative survival and is an independent prognostic factor for poor outcome in GC. |
| Description: |
circNF1 is up-regulated in gastric cancer and is associated with poor prognosis. It promotes migration/EMT and lung metastasis by binding IGF2BP1 to stabilize HMGA2 mRNA, and its expression is increased via ZNF460-driven NF1 transcription. |
| Confidence score: |
0.8726 |