circRNA basic information
circBase ID: hsa_circ_0064288
Name: hsa_circ_ATG7
Synonym: circ_0064288 / circRNA_103285
Host Gene: ATG7
Genomic location(hg19): chr3:11348416-11350535:+
Genomic location(hg38): chr3:11306942-11309061:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; paracancerous tissues / adjacent liver tissues

Cell lines:

Huh7; Hep3B; HCCLM3; MHCC97-L; THLE-3; HEK-293 T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1
Associated microRNA: miR-335-5p
Biological function: promotes HCC cell growth/proliferation and migration
Molecular mechanism: ceRNA sponge of miR-335-5p to upregulate ROCK1
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes)

Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; Clinical Sample Validation; Survival Analysis

Clinical significance:

High circ_0064288 expression was associated with shorter overall survival time of the HCC patients; higher expression of circ_0064288 was associated with larger tumor size

Description:

circ_0064288 is up-regulated in hepatocellular carcinoma and promotes proliferation and migration. It mainly localizes to the cytoplasm and acts as a ceRNA sponge for miR-335-5p, thereby increasing ROCK1 expression. High circ_0064288 expression is associated with shorter overall survival in HCC patients.

Confidence score:

0.7474

Other information
Title:

Circ_0064288 acts as an oncogene of hepatocellular carcinoma cells by inhibiting miR-335-5p expression and promoting ROCK1 expression.

Journal: BMC cancer
Published: 2022
PubMed ID: 35287604
Study type:

combined biological and clinical study

Data availability: GSE94508; GSE97332
Code availability: -