circRNA basic information
circBase ID: hsa_circ_0000390
Name: hsa_circ_FGD4
Synonym: circFGD4
Host Gene: FGD4
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

human GC tissues; paired noncancerous tissues; xenograft tumours; lung metastatic nodules
Cell lines:

MKN28; AGS; SGC7901; MKN45; MGC803; BGC823; HEK-293T; GES-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: APC, beta-catenin, AGO2
Associated microRNA: miR-532-3p
Biological function: suppresses GC cell viability, colony formation, DNA synthesis, migration, EMT, tumorigenesis and metastasis
Molecular mechanism: circFGD4 acts as a miR-532-3p sponge, enhances APC expression, and inactivates beta-catenin/Wnt/beta-catenin signalling
Biological pathway or process:

Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Cohort Study
Clinical significance:

Low circFGD4 expression was correlated with poor tumour differentiation, lymphatic metastasis, and poor prognosis; circFGD4 may serve as a novel biomarker and therapeutic strategy for GC.
Description:

circFGD4 is a human FGD4-derived circRNA that is down-regulated in gastric cancer tissues and cell lines. It functions as a tumor suppressor by sponging miR-532-3p, increasing APC expression, and inactivating beta-catenin/Wnt/beta-catenin signaling, thereby inhibiting GC proliferation, migration, EMT, tumorigenesis and metastasis. Clinically, low circFGD4 expression is associated with poor differentiation, lymphatic metastasis and poor prognosis, supporting its potential as a GC biomarker and therapeutic target.
Confidence score:

0.8961
Other information
Title:

Circular RNA circFGD4 suppresses gastric cancer progression via modulating miR-532-3p/APC/beta-catenin signalling pathway.

Journal: Clinical science (London, England : 1979)
Published: 2020
PubMed ID: 32633323
Study type:

combined biological and clinical study
Data availability: GSE100170; GSE131414; All the data and materials supporting the conclusion of the present study have been included within the article.
Code availability: -