circRNA basic information
circBase ID: hsa_circ_0000654
Name: hsa_circ_CHD2
Synonym: circ_0000654
Host Gene: CHD2
Genomic location(hg19): chr15:93480747-93482948:+
Genomic location(hg38): chr15:92937517-92939718:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma / ESCC
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; matched normal esophageal tissues; serum samples
Cell lines:

TE-1; ECA-109; KYSE-450; HET-1A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F3
Associated microRNA: miR-375
Biological function: promotes cell proliferation and glycolysis; inhibits apoptosis; promotes tumor growth
Molecular mechanism: ceRNA mechanism: circ_0000654 sequesters miR-375 to modulate E2F3 expression
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); glycolysis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Western Blot; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

targeting circ_0000654 and its pathways may have the potential to improve ESCC outcome
Description:

circ_0000654 is up-regulated in ESCC tissues, serum, and cell lines, and functions as an oncogenic circRNA. It mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-375 to increase E2F3, thereby promoting proliferation and glycolysis while inhibiting apoptosis, and enhancing xenograft tumor growth.
Confidence score:

0.7944
Other information
Title:

A novel circ_0000654/miR-375/E2F3 ceRNA network in esophageal squamous cell carcinoma.

Journal: Thoracic cancer
Published: 2022
PubMed ID: 35790503
Study type:

combined biological and clinical study
Data availability: The data sets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -