| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
Suppresses CRC cell proliferation; circ_0002138 knockdown promoted proliferation and colony formation in RKO and HT29 cells. |
| Molecular mechanism: |
circ_0002138 is a stable exonic circRNA preferentially localized in the cytoplasm; tested miRNA sponge candidates did not affect luciferase reporter activity, suggesting circ_0002138 may not function as a sponge and may act through other roles such as RNA-binding protein sequestering or transcription regulation, which needs further study. |
| Biological pathway or process: |
proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0002138 expression could serve as a biomarker for distinguishing CRC tissue from adjacent normal colorectal tissue; AUC = 0.7249, sensitivity = 62.86%, specificity = 74.29%; circ_0002138 expression was significantly correlated with age. |
| Description: |
circ_0002138/hsa_circ_0002138 is a human USP3-derived circRNA that is significantly down-regulated in colorectal cancer tissues. It preferentially localizes in the cytoplasm, is resistant to RNase R, and suppresses CRC cell proliferation in vitro. Its expression shows diagnostic potential for distinguishing CRC tissues from adjacent normal tissues, but the exact molecular regulatory axis remains unresolved. |
| Confidence score: |
0.8294 |