| Expression pattern: |
UP |
| Associated gene: |
YWHAZ, RBM45, SDHB |
| Associated microRNA: |
miR-328-3p |
| Biological function: |
promotes lung cancer cell proliferation, migration, invasion, EMT, xenograft tumor growth, mitochondrial oxidative phosphorylation and succinate metabolism; increases cisplatin resistance; inhibits apoptosis |
| Molecular mechanism: |
ceRNA sponging miR-328-3p to upregulate YWHAZ and activate AKT signaling; binding RBM45 to stabilize SDHB mRNA and enhance mitochondrial oxidative phosphorylation/succinate metabolism |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); oxidative phosphorylation (promotes); mitochondrial function (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; ELISA; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
circ0515 could serve as a potential and effective diagnostic biomarker; higher circ0515 expression had significantly shorter overall survival |
| Description: |
circ0515 is up-regulated in lung cancer/LUAD and promotes proliferation, migration/invasion and xenograft growth. Mechanistically it sponges miR-328-3p to de-repress YWHAZ and activate PI3K/AKT signaling, and it binds RBM45 to stabilize SDHB mRNA, enhancing mitochondrial oxidative phosphorylation/succinate metabolism and cisplatin resistance. |
| Confidence score: |
0.8901 |