circRNA basic information
circBase ID: -
Name: hsa_circ_HIPK3
Synonym: circHIPK3 / circRNA HIPK3
Host Gene: HIPK3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004847
MONDO name: senile cataract
Disease details: age-related cataract / ARC
Disease DO ID:
 9669
Disease MeSH ID:
 -
Disease NCIt ID:
 C35012
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

lens capsules; lens epithelium samples; ARC lens capsules; transparent lens capsules
Cell lines:

-
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: CRYAA
Associated microRNA: miR-193a, miR-193a-3p
Biological function: circHIPK3 regulates HLECs viability, apoptosis, proliferation and EMT; its silencing accelerates apoptosis under oxidative stress and decreases cell viability and proliferation.
Molecular mechanism: circHIPK3 regulates HLECs function through the miR-193a-mediated CRYAA regulatory network.
Biological pathway or process:

apoptosis (inhibits); proliferation (promotes); EMT (promotes); ceRNA regulation (other); mitochondrial function (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; circRNA-seq; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Luciferase Reporter Assay; Western Blot
Clinical significance:

circHIPK3 is down-regulated in all cortical, nuclear and posterior subcapsular subtypes of ARC and may provide insight into ARC pathogenesis.
Description:

circHIPK3 is significantly down-regulated in ARC lens capsules, including cortical, nuclear and posterior subcapsular ARC. Functionally, circHIPK3 supports HLEC viability and proliferation, inhibits oxidative stress-induced apoptosis, and promotes EMT-related marker changes. Mechanistically, it regulates HLEC function through the circHIPK3/miR-193a-3p/CRYAA axis, suggesting a role in cataractogenesis.
Confidence score:

0.7754
Other information
Title:

Circular RNA HIPK3 regulates human lens epithelial cells proliferation and apoptosis by targeting the miR-193a/CRYAA axis.

Journal: Biochemical and biophysical research communications
Published: 2018
PubMed ID: 29959922
Study type:

combined biological and clinical study
Data availability: Supplementary Table 2; https://doi.org/10.1016/j.bbrc.2018.06.149
Code availability: -