| Expression pattern: |
UP |
| Associated gene: |
FUS, XIAP |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion; promotes EMT; promotes tumor growth |
| Molecular mechanism: |
circ0005276 interacts with the RBP FUS and promotes XIAP transcription by facilitating FUS binding to the XIAP promoter, thereby increasing XIAP expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; IF (Immunofluorescence); Luciferase Reporter Assay; ChIP / ChIP-seq; In Vivo Animal Model; Bioinformatics Analysis; circRNA-seq; Clinical Sample Validation |
| Clinical significance: |
Higher circ0005276 expression was observed in PCa tissues with advanced stage and metastasis; proposed as a potential therapeutic marker in PCa. |
| Description: |
circ0005276 is up-regulated in prostate cancer tissues and cell lines and promotes proliferation, migration/invasion, EMT, and in vivo tumor growth. Mechanistically, circ0005276 binds the RBP FUS and enhances FUS-dependent transcriptional activation of its host gene XIAP by promoting FUS binding to the XIAP promoter. |
| Confidence score: |
0.862 |