| Expression pattern: |
UP |
| Associated gene: |
EEF2 |
| Associated microRNA: |
miR-187-3p |
| Biological function: |
Promotes Parkinson's disease progression and neuronal apoptosis; increases oxidative stress and inflammatory factors; knockdown alleviates symptoms. |
| Molecular mechanism: |
Acts as a ceRNA to sponge miR-187-3p, releasing repression of EEF2; validated by Ago2-RIP and dual-luciferase reporter assays. |
| Biological pathway or process: |
ceRNA regulation (promotes); apoptosis (promotes); inflammation (promotes); mitochondrial function (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Bioinformatics Analysis; RNA-seq; RT-qPCR; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Lentiviral Transduction; Annexin V/PI Flow Cytometry; Western Blot; ELISA; IHC (Immunohistochemistry); H&E Staining; TUNEL; In Vivo Animal Model |
| Clinical significance: |
Circulating circFTO levels may be used as an early diagnostic biomarker to assess PD risk level and disease severity. |
| Description: |
circFTO (Hsa_Circ_0105596) is up-regulated in Parkinson's disease and promotes disease-associated neuronal injury. Mechanistically, it sponges miR-187-3p, weakening miR-187-3p-mediated repression of EEF2, thereby enhancing apoptosis and oxidative/inflammatory stress; circFTO knockdown alleviates PD phenotypes in 6-OHDA models. |
| Confidence score: |
0.7302 |