| Expression pattern: |
DN |
| Associated gene: |
WWP2, HOXD13, CTNNB1 |
| Associated microRNA: |
- |
| Biological function: |
inhibits stemness; inhibits EMT; inhibits migration and invasion; inhibits metastasis; suppresses tumor growth |
| Molecular mechanism: |
acts as a scaffold to promote WWP2-HOXD13 interaction, enhancing HOXD13 ubiquitination and proteasomal degradation; reduces HOXD13 binding to the CTNNB1 promoter, suppressing Wnt/beta-catenin signaling |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); EMT (inhibits); stemness (inhibits); metastasis (inhibits); migration (inhibits); invasion (inhibits); ubiquitination (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Western Blot; Wound Healing Assay; Transwell Assay; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
downregulated circGIGYF1 is linked to poor survival rate in CRC patients |
| Description: |
In colorectal cancer, circGIGYF1 is down-regulated and its low expression is associated with worse overall survival. Functionally, circGIGYF1 suppresses stemness, EMT, and metastasis. Mechanistically, it acts as a scaffold that enhances WWP2-HOXD13 interaction to promote HOXD13 ubiquitination/degradation, reducing HOXD13-driven CTNNB1 transcription and inhibiting Wnt/beta-catenin signaling. |
| Confidence score: |
0.8988 |