| Expression pattern: |
UP |
| Associated gene: |
TLR2, CPEB4, ZC3H12D |
| Associated microRNA: |
hsa-miR-6818-3p, hsa-miR-7975, hsa-miR-384, hsa-miR-670-3p |
| Biological function: |
associated with disease severity (ICU length of stay) |
| Molecular mechanism: |
Putative miRNA sponge and putative RBP interactions; possible RBP-facilitated biogenesis via CPEB4/TLR2 binding to flanking introns. |
| Biological pathway or process: |
ceRNA regulation (other); Wnt/beta-catenin (other); Hedgehog (other); immune regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circASPH(2,3,4) level at ICU admission positively correlates with the length of stay in ICU and is proposed as an indicator of illness severity. |
| Description: |
circASPH(2,3,4) is significantly up-regulated in septic patient PBMCs at ICU admission versus ICU discharge, and its level at ICU admission positively correlates with ICU length of stay, suggesting potential utility as a severity indicator. Predicted interactions include binding to specific miRNAs and RBPs (TLR2/CPEB4/ZC3H12D), but these are based on in silico analyses. |
| Confidence score: |
0.6548 |