circRNA basic information
circBase ID: -
Name: hsa_circ_PDHX
Synonym: circRNA pyruvate dehydrogenase complex component X / circPDHX
Host Gene: PDHX
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tumor tissues; adjacent normal tissues; tumor tissues
Cell lines:

PC3; 22RV1; RWPE1; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ACSL1
Associated microRNA: miR-497-5p
Biological function: CircPDHX promotes PCa cell proliferation, migration, fatty acid metabolites and tumor growth, while suppressing apoptosis.
Molecular mechanism: CircPDHX acts as a miR-497-5p sponge to regulate ACSL1 in PCa.
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); lipid metabolism (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

CircPDHX is upregulated in prostate cancer tissues and cells and functions as an oncogenic circRNA. Its knockdown suppresses PCa cell proliferation, migration, fatty acid metabolism and xenograft tumor growth, while promoting apoptosis. Mechanistically, circPDHX sponges miR-497-5p to regulate ACSL1.
Confidence score:

0.8299
Other information
Title:

CircPDHX promotes prostate cancer cell progression in vitro and tumor growth in vivo via miR-497-5p/ACSL1 axis.

Journal: Biochemical and biophysical research communications
Published: 2022
PubMed ID: 35777132
Study type:

combined biological and clinical study
Data availability: Supplementary data to this article can be found online at https://doi.org/10.1016/j.bbrc.2022.06.012; The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -